Lactase Enzyme Essay

This lab go forth examine the specificity of an enzyme (lactase) to a specific substrate (lactose). Students will observe the actions of the enzyme and how cast is important to enzyme answers. Background instruction Lactose, the sugar lay down in take out, is a disaccharide composed of glucose and galactose (both six-sided sugars). Sucrose, ordinary table sugar, is besides a disaccharide composed of fructose and glucose. Glucose is a six-sided sugar and fructose is a five-sided sugar. Lactase is an enzyme that sunders lactose down into galactose and glucose.Lactase can be purchased in pill form by people who are lactose intolerant. These people lack the enzyme, lactase, and can non break down the sugar lactose into its fraction parts. Although lactose is similar to sucrose, lactase will break down sole(prenominal) lactose because of the shape of the sugar. In this lab, you will see lactase break lactose down into galactose and glucose. You will also observe what happens if the shape of lactase is changed due to heating. Materials/ resources * Lactase tablets these can be bought in either(prenominal) drug store or grocery store.Fifteen ccs of milk any milk will work. Water used for change state the lactase tablet, breakup the sucrose and boiling the lactase. * Sucrose five grams per group. * One hundred millilitre graduated cylinder/ten milliliter graduated cylinder measuring stick water and enzyme amounts. * Three four hundred milliliter beakers used for dissolving the lactase tablet, dissolving the sucrose and boiling the lactase. * Five runnel metros these vacuum tube-shaped structures tick the different firmness mixtures. * trial tube rack hold the try on tubes. * Marking pencil mark the try out tubes so that confusion does not occur. * Clock * Hot plate with a Pyrex test tube for denaturing the enzyme. Glucose test strips these strips can be found in any drugstore.Stirring celestial pole helps to mix up the lactase tablet in the water. Lab procedures 1. come across the materials. 2. Label the test tubes with the following labels 0 A. Test tube with read milk and enzyme final result. B. Test tube with skim milk and water. C. Test tube with skim milk and denatured enzyme answer. D. Test tube with sucrose solution and enzyme solution. E. Test tube with sucrose solution and water. 3. In test tube A add ii milliliters of skim milk and star milliliter of enzyme solution. 4. Time for two minutes and test for glucose with the glucose test tape.Record this selective information in table 1. If there was glucose present mark a + in the table. If glucose was absent, mark a - in the table. 5. In test tube B add two milliliters of skim milk and one milliliter of water. 6. arrogate stones throw 4. 7. In test tube C add two milliliters of skim milk and one milliliter of denatured enzyme solution. 8. Repeat step 4. 9. In test tube D add two milliliters of the sucrose solution and one milliliter of enzyme solution. 1 0. Repeat step 4. 11. In test tube E add two milliliters of the sucrose solution and one milliliter of water. 12. Repeat steps 4. Results Glucose Presence in the Following Solutions typeface of Solution Positive or Negative Glucose Result Test tube A milk and enzyme solution   Test tube B milk and water   Test tube C milk and denatured enzyme solution   Test Tube D sucrose solution and enzyme solution   Test Tube E sucrose solution and water   Conclusion and Questions 1. draw and describe the lactose and lactase reaction (whitethorn need to research this). 2. wherefore did the enzyme react to lactose but not to sucrose? 3. What happened when the enzyme was boiled? 4. another(prenominal) way to affect the enzyme is by lowering the pH of the solution. However, lactase is suppose to be able to work in the stomach.Would lowering the pH of the enzyme solution affect the enzyme? Why or why not? 5. What type of reaction is this? Dehydration or hydrolysis? Content to be covered in shutdown paragraphs It should explain the general conclusions of the lab. May refer back to background information to help explain the conclusion of the lab. Should refer back to the GENERAL information in the results. Restate the purpose and state whether the hypothesis was correct or not and why. Clearly state and explains how at least THREE errors may have occurred in the lab. Also explains common pitfalls a person SHOULD avoid.